LOCUS pcDNA3.1(+) Drp1 7481 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pcDNA3.1(+) Drp1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7481)
TITLE Mouse Drp1
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7481)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7481
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 863..882
/label=T7
/note="T7 promoter, forward primer"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(3072..3089)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 3078..3302
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 3348..3776
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3435..3454)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3645..3666
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(3785..3805)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 3790..4119
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3970..4105
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4032..4051
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 4186..4980
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4240..4259)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4850..4869
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 5154..5275
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5191..5210)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5245..5264
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(5324..5340)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5324..5340)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5337..5359)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5348..5364
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5372..5402)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5417..5438
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5555..5572)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5726..6314)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5806..5825)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6485..7345)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7108..7127
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7346..7450)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttggta ccgagctcgg atccatggag gcgctgatcc cggtcatcaa
961 taagctgcag gacgtcttca acacagtggg tgcggacatc atccagctgc ctcagatcgt
1021 cgtagtggga acgcagagca gtgggaagag ctcagtgctg gaaagcctag tgggcaggga
1081 ccttcttccc agaggaactg gtgtggtcac ccggagacct ctcattctgc agctagtcca
1141 cgtttcacca gaagataaaa gaaaaacaac aggagaagaa aatggagttg aagcagaaga
1201 atggggtaaa tttcttcaca ccaaaaacaa gctttacaca gattttgatg aaattcgaca
1261 agaaattgaa aatgaaactg aaagaatttc aggaaataat aagggggtaa gccctgagcc
1321 aatccatctc aaggttttct cgcccaacgt tgtcaacctg acacttgtgg atttaccggg
1381 aatgaccaaa gtacctgtag gcgatcagcc caaggacatc gagcttcaga tcagagaact
1441 tattcttcgg ttcatcagta atcccaattc cattatcctc gccgtcactg ctgcaaatac
1501 agatatggca acatcagaag cactcaagat ttccagagag gtagatccag atgggcgcag
1561 aactctagct gtaatcacta aacttgatct catggatgcg ggtactgatg ccatggatgt
1621 attgatggga agggttattc cagtcaagct tggaataatt ggagtagtta acagaagcca
1681 actggatatt aacaataaga agagtgtaac tgattcaatc cgtgatgagt atgcttttct
1741 tcaaaagaag tacccatctc tggccaacag aaatggaaca aagtatcttg ctaggaccct
1801 gaataggtta cttatgcatc atatcagaga ttgtttacca gagctgaaaa caagaataaa
1861 tgtcttagct gctcagtatc agtctcttct aaatagctat ggtgaaccgg tggatgataa
1921 aagtgctact ttactccagc ttattaccaa atttgccaca gagtattgta acacgattga
1981 aggaaccgca aagtacattg aaacttctga gctatgcggt ggtgctagga tttgttatat
2041 tttccatgag actttcgggc gaaccttaga atctgtggac ccactaggtg gccttaacac
2101 tattgacatc ctgactgcca tcagaaatgc tactggcccc cgtcctgctt tatttgtgcc
2161 tgaagtttca tttgagttac tggtcaaacg tcagattaag cgtctagaag agcccagcct
2221 acggtgtgtg gagctggtcc atgaggagat gcagaggatc attcagcatt gtagcaatta
2281 cagcacacag gaattgttac ggttccctaa acttcacgat gccatagttg aagtagtgac
2341 ctgtcttctt cgtaaaaggt tgcccgtgac aaatgaaatg gtgcataact tagtggcaat
2401 tgagctagcg tatatcaaca caaaacaccc cgactttgct gatgcctgtg ggctaatgaa
2461 caataatata gaggaacaaa gaagaaacag gctagcaaga gagctgcctt cagctggatc
2521 acgggacaag gtcccatctg caggtggtgg gattggagac ggtggtcagg aaccaacaac
2581 aggcaactgg agaggaatgc tgaaaacttc aaaagctgaa gaattacttg ctgaagaaaa
2641 atcaaaacca attccaatta tgccagcaag tccacagaaa ggccatgctg tcaatttgct
2701 agatgtgcca gttccagttg caagaaaact gtctgcccga gaacagcgag attgtgaggt
2761 tattgaaaga cttatcaaat catattttct aattgtcaga aagaatattc aagacagtgt
2821 cccaaaggca gtaatgcatt ttttggttaa tcatgtgaaa gatactcttc agagtgaact
2881 ggtagggcag ctgtataagt catccttatt agatgacctt ctgactgaat ccgaggacat
2941 ggcccaacga agaaaagaag cagcggatat gctgaaggca ttacaaggag ccagtcaaat
3001 tattgctgaa atccgagaga ctcatctttg gtgactcgag tctagagggc ccgtttaaac
3061 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
3121 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
3181 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
3241 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
3301 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
3361 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
3421 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
3481 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
3541 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
3601 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
3661 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
3721 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
3781 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
3841 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
3901 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
3961 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
4021 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
4081 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
4141 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
4201 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
4261 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
4321 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
4381 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
4441 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
4501 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
4561 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
4621 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
4681 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
4741 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
4801 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
4861 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
4921 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
4981 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
5041 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
5101 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
5161 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
5221 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
5281 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
5341 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
5401 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
5461 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
5521 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
5581 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
5641 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
5701 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
5761 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
5821 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
5881 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
5941 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
6001 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
6061 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
6121 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
6181 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
6241 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
6301 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
6361 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
6421 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
6481 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
6541 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
6601 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
6661 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
6721 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
6781 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
6841 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
6901 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
6961 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
7021 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
7081 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
7141 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
7201 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
7261 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
7321 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
7381 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
7441 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
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